کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

بررسی کمی و کیفی فلاونوئیدهای روتین و کوئرستین در میوه گیاه گوجی بری (L.Lycium barbarum) با استفاده از دستگاه HPLC-PDA و تعیین مقدار فنول و فلاونوئید تام عصاره هیدروالکلی آن

نویسندگان :

مهرداد کاشفی¹ فاطمه تاج آبادی² سید وحید قاسمی³ رضا حاجی آقایی⁴ معراج نجفی⁵

1- پژوهشکده گیاهان دارویی جهاد دانشگاهی 2- پژوهشکده گیاهان دارویی جهاد دانشگاهی 3- پژوهشکده گیاهان دارویی جهاد دانشگاهی 4- پژوهشکده گیاهان دارویی جهاد دانشگاهی 5- پژوهشکده گیاهان دارویی جهاد دانشگاهی

کلمات کلیدی :

Goji berry plant, Hydroalcoholic extract, HPLC, Total Phenols and Flavonoids

چکیده :

Abstract Background: Goji berry is a scientific name (Lycium barbarum L.) from Solanaceae family which has been widely used in traditional medicine. Objective: The main objective of this study was to develop a novel method using HPLC to measure the amount of rutin and quercetin flavonoids. Also, considering the reported medicinal properties of leaf and fruit of Lycium barbarum and its commerciality and reports of significant amounts of phenol and flavonoids in the leaves of the plant, the total content of these compounds in the fruit of the plant were also measured (phenols and flavonoids). Results: The results were reported based on HPLC data for the two rutin and quercetin 842 ± 1.2 µg/g plant and 22 ± 1/3 µg/g plant, respectively. The spectrophotometric method was used to determine the total amount of 80% methanolic extract of phenol and flavonoid extract 12.92 ± 1.97 mg Gallic acid/g plant and 3.71 ± 0.56 mg Rutin /g plant, respectively. Conclusion: According to HPLC data, the presence of flavonoids of rutin and quercetin in the hydroalcoholic extract of the fruit of the plant were reported and their quantities were reported for first time. The new HPLC method used for these two compounds showed its effectiveness in the measurements. Determination of total phenol and flavonoid amounts showed significant presence of these compounds.