کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

تأثیر میدان مغناطیسی به عنوان الیسیتور غیر زنده بر رشد و فعالیت های آنتی اکسیدانی میسلیوم قارچ Ganoderma lucidum

نویسندگان :

لیلا مرادی پور¹ وحیده پیام نور²

1- دانشگاه علوم کشاورزی و منابع طبیعی گرگان 2- دانشگاه علوم کشاورزی و منابع طبیعی گرگان

کلمات کلیدی :

key words: Ganoderma lucidum, magnetic field, mycelial growth, antioxidant properties

چکیده :

Ganoderma lucidum, commonly called reishi (Japan) and Ling Zhi (China) is a medicinal fungus, and generally observed on dead trees and timbers of deciduous forest trees such as Quercus castaneifolia, Carpinus betulus, Parrotia persica. Ganoderma lucidum owes its anti-disease properties to presence of secondary metabolites in the fruiting body, mycelium and spores. The aim of present study was to investigate the possibility of increasing the antioxidant activity of Ganoderma lucidum by using a magnetic field as an abiotic elicitor. Mushroom samples were collected from Dr. Bahramnia's Forest Management project- Golestan- Iran. One cm cultured mycelia in PDA medium were exposed to magnetic field with under 20, 40 and 60 milli tesla levels for 30, 60 and 90 minutes. The experiment was conducted as completely randomized design arrangement in factorial with three replications. The growth of the mycelia was measured over ten days. The results showed that there was no significant difference between mycelial growth trends in different treatments. The average growth of treated mycelium was 8.05 mm per day compared to the average growth of 7.4 mm per day for control. According to the results mycelium of Ganoderma lucidum grows "very fast" with a growth of more than 5 cm during 3-4 days. To investigate the effect of magnetic field levels on the antioxidant activities, the grown mycelium were cultured in PDB medium for 14 days and then filtered. The Extracellular methanolic extract was used to evaluate the antioxidant properties by DPPH method. Effect of magnetic field levels on the antioxidant activities of the treatments was significant but it did not make sense at different times (30, 60 and 90 minutes). Antioxidant activities were 21.54, 25.36 and 28.34 for 20, 40 and 60 mili tesla treatments respectively while was 18.56in control. So, according to the results of antioxidant properties, 60 milli tesla in 90 minutes treatment was selected as the best treatment for future experiments about secondary metabolites.