کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / پنجمین کنگره بین المللی و ششمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Quantitative and Qualitative Analysis of Phenolic Content in Walnut Septum Extract with Different Solvents

نویسندگان :

Mahdieh Sayyahi¹ Nader Chaparzadeh² Leila Zarandi Miandoab³

1- دانشگاه شهیدمدنی آذربایجان 2- دانشگاه شهیدمدنی آذربایجان 3- دانشگاه شهیدمدنی آذربایجان

کلمات کلیدی :

Phenol،Septum،Solvent،Walnut

چکیده :

In the last few decades, scientific evidence has highlighted the importance of plants in the prevention and/or supportive treatment of a variety of diseases. Plants contain a significant amount of phenols, which are distributed throughout different parts of the plant and give the plants antioxidant properties. Walnut Septum (WS), a by-product frequently discarded during Walnut processing, contains a phytochemical profile that demonstrates its therapeutic potential. It is Walnut’s main source of polyphenols with powerful antioxidant and anti-inflammatory capacities. However, the detailed information of the compositional profile of phenolics in the Septum remains limited. Thus, to explore the nutraceutical values of the materials, the phenolic profiles of the Walnut Septum must first be identified. To address this question, Walnut Septum extracts were prepared using four different solvents—aqueous, acidic, ethanolic, and methanolic—to assess their efficiency in phenolic compound extraction. The selection of these solvents was based on their distinct chemical properties, influencing solubility and extraction efficiency. Qualitative analyses were conducted, followed by quantitative assessment utilizing the Folin-Ciocalteu Reagent and Spectrophotometry at 765 nm, referencing a standard Gallic acid Calibration curve. The results demonstrated that methanolic and ethanolic solvents yielded the highest phenol extraction, suggesting their superior efficiency in polyphenol extraction. These findings highlight the potential of Walnut Septum as a valuable source of polyphenols, with promising applications in pharmaceutical and nutraceutical formulations. Further studies should explore the bioavailability and therapeutic efficacy of these extracts.