کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Flavonoid constituents and chemotypes of the genus Teucrium L. (Lamiaceae); section Polium from Zagros region, Iran

نویسندگان :

Seyed Hossein Bagheri Moghadam¹ Navaz Kharazian²

1- دانشگاه شهرکرد 2- دانشگاه شهرکرد

کلمات کلیدی :

chemical markers, flavonoid, HPLC-MS/MS, Lamiaceae, Teucrium

چکیده :

The genus Teucrium L. (Lamiaceae family; Ajugoideae sub-family) is a well-known medicinal plant with a rich source of secondary metabolites. The current research attempts to elucidate the flavonoid compounds, chemotypes and specific markers of Teucrium; section Polium. In this section, 29 accessions belonging to three species were collected from center, west, south and southwest of Zagros regions. The total flavonoid was obtained from air-dried leaves using 90% MeOH and purified by CHCl3-MeOH 60:40 and column chromatography. Consequently, nine fractions were analyzed using HPLC-MQ-API-MS/MS. The mass spectrometer was recorded on negative ionization mode. The results of this research showed 30 chemical compounds including flavonoid, quinic acid, and phenylpropanoic acid derivatives. Furthermore, specific flavonoid markers were recognized for each species containing 17 markers for T. capitatum, 6 markers for T. gnaphalodes L.’ Her, and 2 markers for T. polium L. Some of the flavonoid markers were attributed to isorhamnetin-O-di-glucoside (T. polium), myricetin-tetramethylether (T. gnaphalodes), and afzelin-O-di-hexoside (T. capitatum). Moreover, six chemotypes were observed in section Polium, considerably showing the spatial segregation in natural habitats. It was concluded that two geographical regions, namely Isfahan and Boyerahmad had a high chemical divergence. Consequently, the flavonoid constituents identified by HPLC-MS/MS are suitable for pharmaceutical and phytochemical purposes.