کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Co-culture of Ganoderma lucidum with Bacillus subtilis and Aspergillus niger: Optimization of gonodric acid production in bubble column bioreactor

نویسندگان :

Ashrafalsadat Hatamian-zarmi¹ Dana Shakiba² Hale Alvandi³ Zahra Beagom Mokhtari-hosseini⁴ Bahman Ebrahimi-hoseinzadeh⁵

1- دانشگاه تهران 2- دانشگاه تهران 3- دانشگاه تهران 4- دانشگاه حکیم سبزواری 5- دانشگاه تهران

کلمات کلیدی :

Ganoderma lucidum, Ganoderic acids, Secondary metabolites, Taguchi method

چکیده :

Among medicinal fungi, Ganoderma lucidum is one of the most beneficial fungi that has been used in Asian countries for more than two thousand years. G. lucidum is used to treat migraines, high blood pressure, arthritis, gastritis, diabetes, lupus erythematosus, and cardiovascular problems. The metabolites of this fungus have anti-cancer properties and can be used in combination with chemotherapy to treat cancer. The mentioned properties are related to this fungus's secondary metabolites, including terpenoids; Triterpenes are a large group of organic compounds with an aromatic structure, of which ganoderic acids is a member. The low production efficiency of ganoderic acids from G. lucidum has limited its use as a valuable secondary metabolite. Studies show that elicitors increase the production of ganoderic acids in this fungus. Among the elicitors that can stimulate the growth and production of secondary metabolites are volatile microbial organic compounds produced in co-cultures. In this study, the growth of G. lucidum and the production of ganoderic acids in the presence of microbial volatile organic compounds from Bacillus subtilis and Aspergillus niger were investigated. By examining the effect of variables (day and volume of co-culture), the amount of ganoderic acids extracted was 2.9 times higher than the control. The production of ganoderic acids in the bioreactor was then investigated. The effect of initial inoculation percentage, temperature, length to diameter ratio, and aeration rate on ganoderic acids production was studied, and the Taguchi method was selected to optimize these variables. The results showed that the percentage of initial inoculation and temperature had significant effects on biomass production; with a simultaneous increase in temperature and inoculation percentage, biomass production increases. These two variables significantly affect ganoderic acid production, and optimal production condition is 10% of initial inoculation, temperature 25.6 °C, length to diameter ratio of 4.8, and aeration rate of 0.64 vvm. This study shows that microbial volatile organic compounds in the simultaneous microorganism culture can increase the production of ganoderic acids by G. lucidum. Co-cultures could be used to produce other valuable metabolites of G. lucidum, such as medicinal polysaccharides.