کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Evaluation of Antibacterial and Antioxidant effect of Ziziphora tenuior essential oil, native to Zanjan province.

نویسندگان :

Shayan Sadeghilar¹ Amirsaleh Karimi² Alireza Yazdinezhad³ Mehran Mohseni⁴

1- دانشگاه علوم پزشکی زنجان 2- دانشگاه علوم پزشکی زنجان 3- دانشگاه علوم پزشکی زنجان 4- دانشگاه علوم پزشکی زنجان

کلمات کلیدی :

Antibacterial, Antioxidant, MIC, MBC, phenolic content, DPPH, FRAP, HRSA, Ferrous ion chelation, Zanjan, Ziziphora tenuior.

چکیده :

In the present investigation, the chemical compositions and evaluation of Antibacterial and Antioxidant effect of Ziziphora tenuior essential oil were examined. The chemical compositions analyzed with GC–MS. The antibacterial activity of essential oil of Ziziphora tenuior was tested against Bacillus subtilis, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Klebsiella pneumonia by agar well diffusion method, Minimum inhibitory concentrations (MICs) and Minimum bactericidal concentration (MBC). Ziziphora tenuior had several level of antibacterial activities (MIC>0.625 µg/mL & MBC>1.25 µg/mL). Antioxidant activity of essential oil of Ziziphora tenuior were determined by total phenolic with 169.33 ± 7.02 GAE mg/g, DPPH- radical-scavenging activities with significant differences (P<0.05) among IC50 798.71 ± 7.91 mg/L comparable to garlic acid (61.20 ± 1.67 mg/L), Ferric reducing antioxidant power assay (FRAP) with significant differences (P<0.05) among Absorption of essential oil comparable to standard (ascorbic acid), Hydroxyl Radical Scavenging Activity (HRSA) with significant differences (P<0.05) among IC50 of essential oil of Ziziphora tenuior (8.01 ± 0.17 mg/mL) comparable to ascorbic acid (0.12 ± 0.005 mg/mL) and Ferrous ion chelation with significant differences (P<0.05) among IC50 of essential oil of Ziziphora tenuior (79.54 ± 2.98 µg/mL) comparable to EDTA (0.72 ± 0.015 µg/mL). Ziziphora tenuior in all determining methods had several level of antioxidant and antibacterial activities.