کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / پنجمین کنگره بین المللی و ششمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

بررسی کارایی سویه‌های مختلف اگروباکتریوم ((A. rhizogenes و انواع ریز نمونه‌ بر بازده تراریختی ریشه‌های مویین گیاه دارویی سنبل‌الطیب (Valeriana officinalis L)

نویسندگان :

راضیه بیگلری فراش¹ عزیزاله خیری² زهرا قهرمانی³

1- دانشگاه زنجان 2- دانشگاه زنجان 3- دانشگاه زنجان

کلمات کلیدی :

Molecular analysis،Transformation،Tissue culture،Valerian

چکیده :

Valerian )Valeriana officinalis L.( is a medicinal plant whose root is effective in treating depression and Alzheimer's. The aim of this study is to optimize the production of hairy roots in this plant using different strains of Agrobacterium rhizogenes and various microprobes with the aim of producing secondary metabolites under in vitro conditions. This experiment was carried out as a factorial experiment in a completely randomized design with three replications. The factors studied included explants with three levels (leaf, stem and root) and the bacterial strain with 4 levels (A4 and A13, MSU440 and ATCC15834). The results showed that the interaction of the factors studied on the traits of days to hairy root induction, percentage of hairy root induction, number and dry weight of hairy roots after two months was significant. The highest induction percentage (99.3%) and number of days to hairy root emergence (4) and the highest number of roots (7) were in the treatment combination of root explants inoculated with the MSU440 strain. The highest root dry weight was in the treatment combination of leaf microsample and ATCC15834 strain and was 0.2 g. The lowest value (0.02) occurred in the stem microsample and A13 strain. The transgenic nature of hairy roots was confirmed by PCR detection of a part of the rolB gene, while it was not amplified for the natural and non-transgenic roots of the relevant product. In general, the results of this study showed that hairy root induction in Valerian was affected by the explant and bacterial strain and these results are an important prerequisite for experiments related to hairy root culture with the aim of producing secondary metabolites.