کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Piper cubeba fruit extract has cytotoxic effect on normal cells

نویسندگان :

Tayebeh Mohammadi¹ Mehrdad Pooyanmehr² Ali Maleki³ Farhang Tafaroji⁴

1- دانشگاه رازی 2- رازی 3- علوم پزشکی کرمانشاه 4- دانشگاه رازی

کلمات کلیدی :

Piper cubeba, HUVEC, MTT, Viabilty, Apoptosis.

چکیده :

Piper cubeba or tailed pepper fruit is an antioxidant rich herbal source. So its effects on cancer cell types has been studied. In this study, its hydroalcoholic extract effect on human umbilical vein endothelial cell(HUVEC) as normal cells was examined. The cells were treated with increasing concentrations of extract of fruit for 24 h to determine IC50. Cell bio-viability was determined by MTT assay. Thereafter, the cells were treated with IC50 concentration for 3 and 5 days. Cell morphology and apoptosis were examined by Giemsa and acridine orange-ethidium bromide(AO/EB) staining. IC50 was determined. The extract reduced cell viability as a dose and time dependent manner significantly(P<0.05). It changed cell morphology. Apoptotic changes were observed in nuclei. It seems the hydroalcoholic extract of Piper cubeba fruit has cytotoxic effect on normal cells and induces apoptosis which should be considered as a side effect.