کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / چهارمین کنگره بین المللی و پنجمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

ساخت نانوحامل مبتنی بر نانوذرات آهن پارامغناطیسی بیو سنتز شده برای اهداف انتقال ژن

نویسندگان :

سارا عابدینی¹ شهرام پورسیدی² جعفر ذوالعلی³ روح الله عبئالشاهی⁴

1- دانشگاه شهید باهنر کرمان 2- دانشگاه شهید باهنر کرمان 3- دانشگاه شهید باهنر کرمان 4- دانشگاه شهید باهنر کرمان

کلمات کلیدی :

Functionalized iron oxide nanoparticles،Nanoparticles green synthesis،Nanoparticles surface functionalization،Plant biotechnology،Plant transformation

چکیده :

Plant genetic engineering faced by challenges of biomolecules transfer in plant cells with the multi-layered and hard cell wall. Traditional biomolecules transfer approaches have serious limited. Advances in nanotechnology and nanoparticles have created promising to plant species-independent transfer of DNA, RNA, and proteins passively through cell wall. In this research, Superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized in Catharanthus roseus leaf extracts that a rich medicinal plant in secondary metabolites. To characterized and confirmed the production of green synthesized SPIONPs TEM, XRD, FTIR, DLS, and VSM analyses, were done. The green synthesized SPIONs showed a single peak at 242 nm and spherical shape with 9.8 nm size. For nanocarrier organize, the green synthesized SPIONPs were successfully functionalized with a cationic polymer. This DNA@SPIONs could applied in many fields such as gene delivery to plant cells.