کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / چهارمین کنگره بین المللی و پنجمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Advances and Perspectives in Tissue Culture, Genetic Engineering and Biotechnology of Cannabis: An important medicinal plant

نویسندگان :

Mohsen Hesami¹

1- University of Guelph

کلمات کلیدی :

Cannabis،Tissue culture،Genetic engineering،Biotechnology،Cannabinoids،synthetic biology،CRISPR،Machine learning

چکیده :

Cannabis sativa, originating from temperate Asia (e.g., Iran, Afghanistan, China) has been widely used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In this presentation, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches such as machine learning, CRISPR methods such as CRISPR/Cas9-equipped Agrobacterium-mediated genome editing and hairy root culture, that can help improving gene transformation and plant regeneration as well as enhancing secondary metabolite production, will be highlighted and discussed.