کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / چهارمین کنگره بین المللی و پنجمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Isolation, sequencing and bioinformatics studies of the promoter of germacrine D synthase gene in basil

نویسندگان :

Fatemeh Khakdan¹ Sargol Gohari²

1- دانشگاه سمنان 2- دانشگاه سمنان

کلمات کلیدی :

Basil،GerS gene،promoter،regulatory and functional active elements

چکیده :

Basil (Ocimum basilicum L.) is an aromatic plant that is used as a medicinal plant in traditional Iranian medicine. Today, researchers use metabolic engineering approaches as a promising field to increase rare compounds in plants. For this purpose, it is necessary to have a correct understanding of the gene expression process of biosynthetic pathways and how to regulate them. Identifying and sequencing specific promoters and examining them from a practical point of view is one of the most important goals of gene expression studies. Among the terpenoids, the volatile sesquiterpene compound Germacrine D is formed, which is very important in the mechanism of plant tolerance against various environmental stresses due to its defensive effects. Germacrine D is mainly derived from the mevalonate pathway and is processed by Germacrine D synthase (GerS) is synthesized. In order to study how to regulate the GerS gene expression pattern in different environmental conditions, the isolation, characterization and in silico analysis of the pObGerS promoter was also performed. The results showed that in the promoter of this gene There are a large number of motifs responding to light and environmental conditions, several regulatory elements such as TATA and CAAT boxes. Also, motifs responding to light, motifs responding to heat, drought and abiotic factors were also identified in the obtained sequence. These results can help to understand how germacrene D synthesis is regulated and facilitate its metabolic engineering.