کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Green root fabrication and chitosan encapsulation of Cerium Oxide Nanoparticles as antibacterial agent

نویسندگان :

Nosheen Akhter¹ Parichehr Hanachi²

1- دانشگاه الزهرا(س) 2- دانشگاه الزهرا(س)

کلمات کلیدی :

Cerium oxide nanoparticles، Green synthesis، Arctium lappa l ، Chitosan encapsulation, Novel synthesis, Antimicrobial activity

چکیده :

Green synthesis method is gaining recognition for the production of Cerium nanoparticles (Ce-NPs) due to their multiple applications. The most important applications of CeNPs used, as an anti-bacterial agent. The use of plant in the synthesis of nanoparticles emerges as a cost effective and eco-friendly approach. In the present study, Arctium lappa l. root was used to synthesize Cerium nanoparticles (Ce NPs). These were encapsulated with nano-chitosan by sol gel method. The plant Arctium lappa l. contain following active reagents (Ethanol, Acetone, 1.Propanol, 1-Propanol, 2methy- , Ethane, 1,1, diethoxy , 1-Butanol,3-methyl- ,Oleic Acid) elements act as a reducing agent for the synthesis of CeO2NPs. NPs produced were analyzed by Fourier transform-IR spectroscopy shows the functional group of Ce-O and Chitosan (Cs) encapsulated Ce shows the C-O-C stretching bond. X-ray diffraction analysis SEM and EDX techniques were also used to morphologically investigate the particles’ crystal structure and purity of sample. Staphylococcus aureus and Pseudomonas aeruginosa were utilized to evaluate the antibacterial activity of the CeNPs. SEM showed the formation of Ce NPs mono dispersed within the size range of 20.08 nm. X-ray diffraction analysis showed that the cubic crystalline structure of the nanoparticles having size of 28 nm. After encapsulation by chitosan nanoparticles the size of CeO2NPs enhances to 56 nm thus making average of about 28 nm. The results of the disc diffusion test used for measuring the anti-bacterial activity of the synthesized Cerium nanoparticles indicated that the formed nanoparticles possessed a suitable anti-bacterial activity. The more interestingly our results of Encapsulated Cerium Oxide nanoparticles enhance the antibacterial activity against all the test strains in comparison with pure CeO2NPs. Biologically synthesized cerium oxide nanoparticles can be used in pharmaceutical industry to combat MDR strains.