کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / پنجمین کنگره بین المللی و ششمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Phytochemical and Enzymatic Responses of In Vitro Hypericum perforatum L. Seedlings to Chitosan Treatment

نویسندگان :

Mojgan Soleimanizadeh¹ Somayeh Karimi Takallo²

1- University of Hormozgan 2- University of Hormozgan

کلمات کلیدی :

antioxidant enzymes،secondary metabolites،in vitro culture،chitosan elicitor،Hypericum perforatum L.

چکیده :

Hypericum perforatum L. is a medicinal plant rich in secondary metabolites with well-documented pharmacological effects. In vitro culture, combined with elicitor application, presents a promising strategy to enhance the production of these bioactive compounds. This study aimed to evaluate the effects of chitosan elicitation on the phytochemical content and antioxidant enzyme activities in H. perforatum seedlings grown under in vitro conditions. Seeds were cultured on MS medium supplemented with four concentrations of chitosan (0, 25, 50, and 75 mM), and the seedlings were maintained under controlled environmental conditions. Phytochemical parameters, including total flavonoid, phenolic, and anthocyanin contents, as well as the activities of catalase (CAT) and peroxidase (POD), were measured using spectrophotometric methods. The results showed that chitosan significantly enhanced all measured phytochemical traits compared to the control, with the 50 mM concentration being the most effective. Total flavonoid and phenolic contents increased markedly, likely due to the upregulation of biosynthetic genes. Similarly, anthocyanin content showed a significant rise, supporting previous reports on chitosan’s stimulatory effects on pigment biosynthesis. Enzymatic assays revealed a substantial increase in CAT and POD activities in response to chitosan, indicating improved antioxidant defense. Overall, chitosan proved to be an effective elicitor for boosting the accumulation of valuable secondary metabolites and enhancing antioxidant enzyme activities in H. perforatum L. under in vitro conditions.