کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / چهارمین کنگره بین المللی و پنجمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Investigating the antioxidant, antimicrobial and antiproliferative activities of Eryngium planum extract against cancer cell line Hela and Enterococcus faecalis

نویسندگان :

Fatemeh Shams Moattar¹ Khosro Issazadeh² Sepideh Esmaeli³ Sahar Doaei⁴

1- دانشگاه آزاد اسلامی واحد لاهیجان 2- دانشگاه آزاد اسلامی واحد لاهیجان 3- دانشگاه آزاد واحد لاهیجان 4- دانشگاه آزاد واحد لاهیجان

کلمات کلیدی :

: Eryngium planum،Enterococcus faecalis،Antimicrobial،Methanolic extract

چکیده :

Plants have been established to be a rich resource of antioxidant and antibacterial compounds and include large amounts of secondary metabolites useful properties of these extracts are related to secondary metabolites such as phenolic, flavonolic, and flavonoid compounds. Eryngium planum is a genus of aromatic plants native to the northern regions of Iran. Some of its medicinal benefits are already known. The purpose of this study is to investigate the effect of Eryngium planum plant extract on Enterococcus faecalis bacteria (causing endocarditis, sepsis, meningitis and urinary and genital tract infections) along with evaluating Hela cell viability in the presence of the extract. After collecting the plant and drying it, methanolic and aqueous extracts in two methods (soaking and soxhelet) were prepared. Since phenolic compounds have multiple effects, the amount of phenolic compounds in the methanolic extract was measured by the Folin-ciocalteu method. Antimicrobial activity was first measured by disk diffusion test, and then MIC and MBC were measured by Microdilution method. Finally, the anti-proliferative activity of plant extracts on cervical cancer cell line (HeLa) was investigated using MTT method. The volume of phenolic compounds of methanolic extract was 901/66mg/ml. In the study conducted, Eryngium planum has a significant amount of phenolic compounds, hence it is a suitable candidate for measuring its antimicrobial and anti-proliferative activity.The investigation of the antimicrobial effect showed that In the case of the methanolic extract isolated by the Soxhlet method, the concentration of 50 mg/ml as MIC and the concentration of 100 mg/ml as MBC, and in the case of the methanol extract of the plant prepared by the soaking method, the concentration of 100 mg/ml as MIC and the concentration of 200 mg/ml as MBC were considered and the results of MTT assay showed that the extract of this plant has no inhibitory effect on HELa cancer cell line.According to the obtained results, the extract of this plant can be a suitable candidate for replacing antibacterial chemical drugs