کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Protective effect of an isolated polysaccharide from Rosa canina in STZ-treated Rin5-F cell line through modulation of cell cycle

نویسندگان :

Soraya Sajadimajd¹ Gholamreza Bahrami² Bahareh Mohammadi³ Alireza Farokhi⁴

1- دانشگاه رازی 2- دانشگاه علوم پزشکی کرمانشاه 3- دانشگاه علوم پزشکی کرمانشاه 4- دانشگاه علوم پزشکی کرمانشاه

کلمات کلیدی :

Polysaccharide, Rin5-F cells, Cell cycle, Bax, Bcl2, cyclin d1

چکیده :

Polysaccharide isolated from medicinal herbs plays an essential role in treatment of metabolic diseases including diabetes. To understand the underlying molecular mechanism of isolated polysaccharide from Rosa canina, modulation of cell cycle was evaluated in a diabetic model, STZ-treated Rin5-F cell line. Herein, polysaccharide was extracted from Rosa canina and evaluated by using infrared radiation (IR), high-performance liquid chromatography diode array detector tandem mass spectroscopy (HPLC-DAD MS/MS) and nuclear magnetic chromatography (NMR) systems. Rin-5F cells were treated with STZ and isolated polysaccharide alone and in combination. The viability of the cells, cell cycle and the expression of Bax, Bcl2 and cyclin d1 were analyzed using MTT assay, flow cytometry and qRT-PCR, and western blotting respectively. Polysaccharide increased the viability of Rin5-F cells exposed upon STZ as post and pre-treatments. Cell cycle analyses indicated the protective effect of isolated polysaccharide through scale down in the sub G1 phase and scale up in S phase. The ratio of Bax/Bcl2 was decreased while the expression of cyclin d1 increased in STZ and polysaccharide co-treated-Rin5-F cells compared to STZ-treated cells. The results indicated that the protective effects of isolated polysaccharide was mediated by the modulation of cell cycle and it is expected that the results paved the way to speculate novel anti-diabetic strategies.