کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Response of chavicol O-methyltransferase gene promoter of Ocimum basilicum to drought stress

نویسندگان :

Fatemeh Khakdan¹ Athar Sadat Javanmard²

1- دانشگاه فرزانگان سمنان 2- دانشگاه یاس

کلمات کلیدی :

chavicol O-methyltransferase gene ،Ocimum basilicum ،gene regulation ،drought stress

چکیده :

Methyl chavicol, a volatile phenylpropenes, is a part of defense system of Ocimum species. The final step in the biosynthesis of methyl chavicol is catalyzed by the chavicol O-methyltransferase (CVOMT) enzyme. Understanding the transcriptional regulation of the gene encoding this rate-limiting enzyme is an important key in metabolite engineering approaches. A 616 bp- upstream fragment of the ObCVOMT gene has been obtained from Ocimum basilicum genome using genome walking technology and cloned into the upstream of the β-glucuronidase (GUS) reporter gene to determine the GUS water stress-specific expression pattern. The transgenic tobacco showed that pObCVOMT drove GUS reporter gene special expression in different water stress levels. The in silico analysis of cis-acting elements also revealed pObCVOMT promoter contained transcription factor-binding sequences involved in transcriptional control in drought- or dehydration-response.