کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / چهارمین کنگره بین المللی و پنجمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Effect of endophytic fungi isolated from Liquorice (Glycyrrhiza glabra) on Candida glabrata in laboratory conditions

نویسندگان :

Melika Esfandiari¹ Reza Habibipour² Mohsen Rajabi³ Farnoosh Rasoul Sharifi⁴

1- گروه میکروب شناسی، دانشکده علوم پایه، واحد همدان ، دانشگاه آزاد اسلامی، همدان، ایران 2- گروه میکروب شناسی، دانشکده علوم پایه، واحد همدان ، دانشگاه آزاد اسلامی، همدان، ایران 3- گروه منابع طبيعي، مركز تحقيقات و آموزش كشاورزي و منابع طبيعي استان همدان، سازمان تحقيقات، آموزش و ترويج كشاورزي، همدان، ايران 4- گروه میکروب شناسی، دانشکده علوم پایه، واحد همدان ، دانشگاه آزاد اسلامی، همدان، ایران

کلمات کلیدی :

Licorice،Endophytic fungi،Candida glabrata،antagonist

چکیده :

Introduction: Licorice (Glycyrrhizaglabra) is a medicinal plant that grows in many regions around the world. This plant has anti-inflammatory, antibacterial, antioxidant and expectorant properties and is effective in detoxification and liver protection. Licorice root is mentioned as medicine in most pharmacopoeias and has been used medicinally in America for about 4000 years and has been registered in China and other countries. Licorice is used to treat gastritis, respiratory infections in traditional Asian and European medicine, and to treat hepatitis, tumor growth, and heart diseases in traditional Chinese medicine. Endophytes are closely related to plant pathogens, but they have limited pathogenicity and probably evolved from plant pathogenic fungi. The symbiosis between plant and endophyte has been proven; In this way, the plant protects and nourishes the endophyte, and the endophyte, in turn, produces plant growth-regulating bioactive substances that increase the growth and strength of the host's competition in nature. Therefore, the antagonistic effect of endophytic fungi from Licorice with human pathogenic fungi was considered. Materials and methods: The plants were collected in summer, 2021 from three different areas of Hamadan province. After surface disinfection, the explants (including stem, leaf and root segments) were cultured on PDA medium. After that, endophytic fungi isolated from Licorice were used and their biocontrol and antagonistic ability was evaluated by cross-culture on PDA against human pathogenic fungi such as Candida glabrata (PFCC =52971) (from Pasteur Institute). Test was performed based on a randomized complete block design with 5 treatments (4 endophytic fungi +control) and nine replications. Statistical analysis was performed by SPSS 20.0 software and means comparing was done by Duncan test at 0.05 level. Results: Results showed endophytic fungi had significant effect on studied Candida glabrata (P<0.01), therefore, Aspergillus niger and Fusarium oxysporum (endophytic fungus) had maximum and minimum effect on this fungus with inhibition percentage of 83.90% and 23.40%, respectively. Also, comparison of means based on Duncan's test showed that Aspergillus niger and Fusarium f.sp had similar effect on C. glabrata and were statistically placed at the same level (with the average radius of fungal growth equal to 25.67 and 25.17 mm, respectively). Conclusion: Endophytic fungi including A. niger and Fusarium f.sp can be promising microorganisms for the future as a biological control and antagonist in studied human pathogenic fungi.