کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

افزایش فعالیت کاسپاز3 و 7 در رده سلولی MDA-MB-231 سرطان سینه تحت تاثیر عصاره متانولی گیاه جاشیر زاگرسی ( Prangos crossoptera)

نویسندگان :

زهرا درخشان هوره¹ مریم موری²

1- دانشگاه آزاد اسلامی واحد شهرکرد 2- دانشگاه آزاد اسلامی واحد علوم و تحقیقات

کلمات کلیدی :

MDA-MB-231, Medicinal plants, Prangos crossoptera, Caspase 3/7

چکیده :

Breast cancer is regarded as a multifactorial disease that leads to many deaths among women. Detrimental side effects, drug resistance, and a high rate of mortality have prompted the need to discover novel, safe, and effective drugs. Medicinal plants as an enormous source of native components are one of the main candidates for discovering new anticancer drugs. Prangos genus is one of the important medicinal plants with valuable therapeutic virtues and anticancer properties of several Prangos species have been reported. This study aimed to evaluate the anticancer properties of the methanolic extract of Prangos crossoptera in the MDA-MB-231 cell line. In the current study, MDA-MB-231 (human breast adenocarcinoma) and HDF (Human Dermal Fibroblasts) cell lines were treated with different concentrations of methanolic extracts of Prangos crossoptera (0, 50, 150, 250, 350, and 450 µg/mL) for 24, 48, and 72 h. Cytotoxicity effects of the samples on the MDA-MB-231 and HDF cells were examined using [3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. Also, caspase 3/7 activity was measured using caspase 3/7 colorimetric assay kit to investigate the effects of the extracts on the programed cell death pathway in the treated cells. The results of the current study revealed that growth of MDA-MB-231 cells significantly inhibited after 48 and 72h of treatment with extract compared to the control group (p < 0.05). Furthermore, measurement of caspase 3/7 activity showed that methanolic extract of Prangos crossoptera significantly increased the caspase 3/7 activity in treated cells compared to the control group. Prangos crossoptera seems to affect the programed cell death pathway by increasing the caspase 3/7 activity in MDA-MB-231 cell line.