کنگره بین المللی زیست فناوری گیاهان دارویی و قارچ های کوهی

صفحه اصلی / پنجمین کنگره بین المللی و ششمین همایش ملی زیست فناوری گیاهان دارویی و قارچ های کوهی

Valorization of Olive Oil Processing By-Products through the Recovery of Bioactive Phenolic Compounds: Evaluation and Comparative Analysis of Antioxidant Assays (DPPH, ABTS, and FRAP)

نویسندگان :

Mahnaz Sharifnasab¹ Mohsen Farhadpour² Mahdi Hosseini Mazinani³

1- National Institute of Genetic Engineering and Biotechnology 2- National Institute of Genetic Engineering and Biotechnology 3- National Institute of Genetic Engineering and Biotechnology

کلمات کلیدی :

Natural Antioxidants،Polyphenols،Olive Pomace،Resin-Based Extraction،Sustainable Valorization

چکیده :

Extraction and purification of polyphenolic compounds from agriculture wastes, especially olive pomace, have been the subject of increasing scientific interest owing to the potent antioxidant, anti-inflammatory, and antimicrobial activities of these compounds. The overall health-promoting activity of olive phenolic components is well established. Over the past few years, the biological activities of two compounds isolated, namely oleuropein and hydroxytyrosol, have been defined with particular emphasis on their antioxidant activity. (1) Of the several methods available, resin-based separation—more specifically, with macroporous resins—has been shown to be a very efficient methodology for selective adsorption, enrichment, and purification of polyphenols owing to their large surface area, chemical inertness, and preference for phenolic moieties(2). Molecularly imprinted resins display a high selectivity in separating target phenolic compounds like hydroxytyrosol and oleuropein, enhancing their bioavailability for food and pharmaceutical applications(3). In this study the extract from olive oil waste was enriched for major phytochemical constituents by using macroporous resins. One of the resins was selected for its optimum efficiency in the enrichment and purification process, and adsorption–desorption of polyphenols was then optimized both under static and dynamic conditions. In the subsequent steps, crucial parameters like type of resin, concentration of sample loading, adsorption/desorption times, and solvent conditions were systematically optimized. These optimizations led to a reproducible and highly efficient protocol for the recovery of polyphenols, in consonance with earlier reports on the effectiveness of macroporous resin-based separation techniques. The antioxidant activity of the crude extract and the enriched fraction was determined using DPPH, ABTS, and FRAP assays. The results indicated that both fractions had a significant free radical scavenging capacity, with the enriched fraction showing much higher activity. Furthermore, based on the lipophilic nature of certain antioxidant constituents present in olive fruit, it was observed that the DPPH assay showed higher sensitivity and effectiveness in detecting antioxidant activity compared to the other methods employed.